whole human genome beadchip h12 microarray Search Results


microarray chips  (CapitalBio Corporation)
90
CapitalBio Corporation microarray chips
Microarray Chips, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
microarray chips - by Bioz Stars, 2026-10
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anti-human ifn-γ (b27  (Becton Dickinson)
90
Becton Dickinson anti-human ifn-γ (b27
DATA AND SOFTWARE AVAILABILITY
Anti Human Ifn γ (B27, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human cd3 (sp34-2  (Becton Dickinson)
90
Becton Dickinson anti-human cd3 (sp34-2
(A–E) ATs from naïve C57BL/6 mice were analyzed by flow cytometry. (A) Representative plots of transcription factor expression or cytokine production by CD8+ or CD4+ T cells from the mAT after PMA/ionomycin restimulation with BFA. (B) Numbers of CD8+ T cells and non-Treg CD4+ T cells from the mAT producing the indicated cytokine. (C) Frequencies of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells after PMA/ionomycin restimulation with BFA isolated from gAT, scAT, and mAT. (D) CD8+ (top) and CD4+ (bottom) T cells isolated from the mAT. Left: representative plots of CD44 and CD62L expression, Right: representative histograms of CD69 expression on CD44+CD62L− (red) or CD44−CD62L+ (blue) T cells. (E) Numbers of central memory (Tcm: CD44+CD62L+), effector memory (Tem: CD44+CD62L−CD69−) and resident memory (Trm: CD44+CD62L−CD69+) CD8+ and CD4+ T cells per gram of gAT, scAT, and mAT. (F) Conjoined pairs of naïve CD45.1 and CD45.2 congenic C57BL/6 mice were analyzed 6 weeks after parabiosis surgery to quantify the origin of CD8+ (left) and CD4+ (right) memory T cell subsets (described in (E)) in the spleen (Spl), mLN, siLP, and mAT. The percentage of cells originating from host (black bars) or donor (white bars) animals is shown. nd not detected. (G–K) mAT isolated from healthy rhesus macaques was analyzed by flow cytometry. (G) Representative plot (gated on <t>CD3+CD8+</t> T cells) indicating the gating strategy for naïve (CD95−CD28lo), central memory or stem cell memory (Tcm/scm) (CD95+CD28+), and Tem/rm (CD95+CD28−) T cells. (H) Representative plots showing CD8+ (left) and CD4+ (right) naïve, Tcm/scm, and Tem/rm T cells. (I) Representative histograms of CD69 expression on CD8+ (left) and CD4+ (right) naïve (shaded) and Tem/rm T cells (blue). (J) Frequencies of CD8+ (left) and CD4+ (right) memory T cell subsets from (HI). (K) Representative plots (gated on <t>CD3+</t> T cells) from concatenation of all samples, showing cytokine production by T cell subsets. Numbers in representative plots indicate mean±SD. In all bar graphs, error bars represent standard deviation. Data are representative of at least 2 experiments with at least 4 mice, 3 pairs of parabiotic animals, or a total of 3 rhesus macaques. See Figure S1.
Anti Human Cd3 (Sp34 2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human cd3 (sp34-2 - by Bioz Stars, 2026-10
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anti-human il-13 (jes10-5a2  (Becton Dickinson)
90
Becton Dickinson anti-human il-13 (jes10-5a2
DATA AND SOFTWARE AVAILABILITY
Anti Human Il 13 (Jes10 5a2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human cd69 (fn50  (Becton Dickinson)
90
Becton Dickinson anti-human cd69 (fn50
(A–E) ATs from naïve C57BL/6 mice were analyzed by flow cytometry. (A) Representative plots of transcription factor expression or cytokine production by CD8+ or CD4+ T cells from the mAT after PMA/ionomycin restimulation with BFA. (B) Numbers of CD8+ T cells and non-Treg CD4+ T cells from the mAT producing the indicated cytokine. (C) Frequencies of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells after PMA/ionomycin restimulation with BFA isolated from gAT, scAT, and mAT. (D) CD8+ (top) and CD4+ (bottom) T cells isolated from the mAT. Left: representative plots of CD44 and CD62L expression, Right: representative histograms of <t>CD69</t> expression on CD44+CD62L− (red) or CD44−CD62L+ (blue) T cells. (E) Numbers of central memory (Tcm: CD44+CD62L+), effector memory (Tem: CD44+CD62L−CD69−) and resident memory (Trm: CD44+CD62L−CD69+) CD8+ and CD4+ T cells per gram of gAT, scAT, and mAT. (F) Conjoined pairs of naïve CD45.1 and CD45.2 congenic C57BL/6 mice were analyzed 6 weeks after parabiosis surgery to quantify the origin of CD8+ (left) and CD4+ (right) memory T cell subsets (described in (E)) in the spleen (Spl), mLN, siLP, and mAT. The percentage of cells originating from host (black bars) or donor (white bars) animals is shown. nd not detected. (G–K) mAT isolated from healthy rhesus macaques was analyzed by flow cytometry. (G) Representative plot (gated on CD3+CD8+ T cells) indicating the gating strategy for naïve (CD95−CD28lo), central memory or stem cell memory (Tcm/scm) (CD95+CD28+), and Tem/rm (CD95+CD28−) T cells. (H) Representative plots showing CD8+ (left) and CD4+ (right) naïve, Tcm/scm, and Tem/rm T cells. (I) Representative histograms of CD69 expression on CD8+ (left) and CD4+ (right) naïve (shaded) and Tem/rm T cells (blue). (J) Frequencies of CD8+ (left) and CD4+ (right) memory T cell subsets from (HI). (K) Representative plots (gated on CD3+ T cells) from concatenation of all samples, showing cytokine production by T cell subsets. Numbers in representative plots indicate mean±SD. In all bar graphs, error bars represent standard deviation. Data are representative of at least 2 experiments with at least 4 mice, 3 pairs of parabiotic animals, or a total of 3 rhesus macaques. See Figure S1.
Anti Human Cd69 (Fn50, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human cd69 (fn50 - by Bioz Stars, 2026-10
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anti-mouse ly-6g (ia8  (Becton Dickinson)
90
Becton Dickinson anti-mouse ly-6g (ia8
DATA AND SOFTWARE AVAILABILITY
Anti Mouse Ly 6g (Ia8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse ly-6g (ia8 - by Bioz Stars, 2026-10
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anti-mouse siglec f (e50-2440  (Becton Dickinson)
90
Becton Dickinson anti-mouse siglec f (e50-2440
DATA AND SOFTWARE AVAILABILITY
Anti Mouse Siglec F (E50 2440, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+beadchip+h12+microarray/hla+dr/pmc05773068-709-217-221
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anti-mouse gata-3 (l50-823  (Becton Dickinson)
90
Becton Dickinson anti-mouse gata-3 (l50-823
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Anti Mouse Gata 3 (L50 823, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse gata-3 (l50-823 - by Bioz Stars, 2026-10
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anti-mouse nk1.1 (pk136  (Becton Dickinson)
90
Becton Dickinson anti-mouse nk1.1 (pk136
(A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or vehicle control (ctrl.) and evaluated by flow cytometry 1 hour post-injection. (A) Representative histogram shows expression of CD69 on YopE:Kb+CD8+ T cells after vehicle control (dotted line) or peptide (gray shading) injection (left) and MFI of CD69 in the indicated organs after the indicated treatment is quantified (right). (B) Representative plots show IFN-γ and TNF-α production by YopE:Kb+CD8+ T cells 1 hour after vehicle control (left) or peptide (right) injection in the indicated organs. Frequencies of IFN-γ+ TNF-α+ YopE:Kb+ CD8+ T cells in the indicated organs are shown in the bar graph. (C) Mice were infected orally with a fluorescent reporter strain of T. gondii. 6 weeks post-infection, mice were injected i.v. with MHC-I and MHC-II peptides or vehicle control and analyzed by flow cytometry 1 hour post injection. Representative plots show IFN-γ and TNF-α in CD44+CD8+ T cells in the indicated organ. Bar graphs show the frequencies of IFN-γ+TNF-α+ cells within the CD44+ CD8+ T cells. (D–E) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69-77 peptide or an equal volume of vehicle control and evaluated by flow cytometry at 1 and 4 hours post-injection. (D) Representative plots show neutrophils in the indicated organs (gated on live CD45+TCRβ−Siglec <t>F−NK1.1−B220−</t> cells) 4 hours after injection. Bar graph shows frequency of neutrophils at 1 and 4 hours post-injection in the siLP and mAT. (E) Representative plots show monocytes (gated on live CD45+TCR-β−Siglec F−NK1.1−B220−CD11b+Ly-6G−CD64+CCR2+ cells) 4 hours after injection in the indicated organs. Bar graph shows the frequency of monocytes at 1 and 4 hours post-injection in the siLP and mAT. Numbers in representative plots indicate mean±SD. Error bars in all bar graphs represent standard deviation and statistics are calculated using one way Anova adjusted for multiple comparisons. Data are representative of at least 2 experiments with 2–6 mice per group. ns not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S5.
Anti Mouse Nk1.1 (Pk136, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+beadchip+h12+microarray/propidium+iodide/pmc05773068-709-210-213
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anti-mouse nk1.1 (pk136 - by Bioz Stars, 2026-10
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anti-mouse γδtcr (gl3  (Becton Dickinson)
90
Becton Dickinson anti-mouse γδtcr (gl3
DATA AND SOFTWARE AVAILABILITY
Anti Mouse γδtcr (Gl3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

(A–E) ATs from naïve C57BL/6 mice were analyzed by flow cytometry. (A) Representative plots of transcription factor expression or cytokine production by CD8+ or CD4+ T cells from the mAT after PMA/ionomycin restimulation with BFA. (B) Numbers of CD8+ T cells and non-Treg CD4+ T cells from the mAT producing the indicated cytokine. (C) Frequencies of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells after PMA/ionomycin restimulation with BFA isolated from gAT, scAT, and mAT. (D) CD8+ (top) and CD4+ (bottom) T cells isolated from the mAT. Left: representative plots of CD44 and CD62L expression, Right: representative histograms of CD69 expression on CD44+CD62L− (red) or CD44−CD62L+ (blue) T cells. (E) Numbers of central memory (Tcm: CD44+CD62L+), effector memory (Tem: CD44+CD62L−CD69−) and resident memory (Trm: CD44+CD62L−CD69+) CD8+ and CD4+ T cells per gram of gAT, scAT, and mAT. (F) Conjoined pairs of naïve CD45.1 and CD45.2 congenic C57BL/6 mice were analyzed 6 weeks after parabiosis surgery to quantify the origin of CD8+ (left) and CD4+ (right) memory T cell subsets (described in (E)) in the spleen (Spl), mLN, siLP, and mAT. The percentage of cells originating from host (black bars) or donor (white bars) animals is shown. nd not detected. (G–K) mAT isolated from healthy rhesus macaques was analyzed by flow cytometry. (G) Representative plot (gated on CD3+CD8+ T cells) indicating the gating strategy for naïve (CD95−CD28lo), central memory or stem cell memory (Tcm/scm) (CD95+CD28+), and Tem/rm (CD95+CD28−) T cells. (H) Representative plots showing CD8+ (left) and CD4+ (right) naïve, Tcm/scm, and Tem/rm T cells. (I) Representative histograms of CD69 expression on CD8+ (left) and CD4+ (right) naïve (shaded) and Tem/rm T cells (blue). (J) Frequencies of CD8+ (left) and CD4+ (right) memory T cell subsets from (HI). (K) Representative plots (gated on CD3+ T cells) from concatenation of all samples, showing cytokine production by T cell subsets. Numbers in representative plots indicate mean±SD. In all bar graphs, error bars represent standard deviation. Data are representative of at least 2 experiments with at least 4 mice, 3 pairs of parabiotic animals, or a total of 3 rhesus macaques. See Figure S1.

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: (A–E) ATs from naïve C57BL/6 mice were analyzed by flow cytometry. (A) Representative plots of transcription factor expression or cytokine production by CD8+ or CD4+ T cells from the mAT after PMA/ionomycin restimulation with BFA. (B) Numbers of CD8+ T cells and non-Treg CD4+ T cells from the mAT producing the indicated cytokine. (C) Frequencies of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells after PMA/ionomycin restimulation with BFA isolated from gAT, scAT, and mAT. (D) CD8+ (top) and CD4+ (bottom) T cells isolated from the mAT. Left: representative plots of CD44 and CD62L expression, Right: representative histograms of CD69 expression on CD44+CD62L− (red) or CD44−CD62L+ (blue) T cells. (E) Numbers of central memory (Tcm: CD44+CD62L+), effector memory (Tem: CD44+CD62L−CD69−) and resident memory (Trm: CD44+CD62L−CD69+) CD8+ and CD4+ T cells per gram of gAT, scAT, and mAT. (F) Conjoined pairs of naïve CD45.1 and CD45.2 congenic C57BL/6 mice were analyzed 6 weeks after parabiosis surgery to quantify the origin of CD8+ (left) and CD4+ (right) memory T cell subsets (described in (E)) in the spleen (Spl), mLN, siLP, and mAT. The percentage of cells originating from host (black bars) or donor (white bars) animals is shown. nd not detected. (G–K) mAT isolated from healthy rhesus macaques was analyzed by flow cytometry. (G) Representative plot (gated on CD3+CD8+ T cells) indicating the gating strategy for naïve (CD95−CD28lo), central memory or stem cell memory (Tcm/scm) (CD95+CD28+), and Tem/rm (CD95+CD28−) T cells. (H) Representative plots showing CD8+ (left) and CD4+ (right) naïve, Tcm/scm, and Tem/rm T cells. (I) Representative histograms of CD69 expression on CD8+ (left) and CD4+ (right) naïve (shaded) and Tem/rm T cells (blue). (J) Frequencies of CD8+ (left) and CD4+ (right) memory T cell subsets from (HI). (K) Representative plots (gated on CD3+ T cells) from concatenation of all samples, showing cytokine production by T cell subsets. Numbers in representative plots indicate mean±SD. In all bar graphs, error bars represent standard deviation. Data are representative of at least 2 experiments with at least 4 mice, 3 pairs of parabiotic animals, or a total of 3 rhesus macaques. See Figure S1.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Flow Cytometry, Expressing, Isolation, Standard Deviation

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

(A–E) ATs from naïve C57BL/6 mice were analyzed by flow cytometry. (A) Representative plots of transcription factor expression or cytokine production by CD8+ or CD4+ T cells from the mAT after PMA/ionomycin restimulation with BFA. (B) Numbers of CD8+ T cells and non-Treg CD4+ T cells from the mAT producing the indicated cytokine. (C) Frequencies of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells after PMA/ionomycin restimulation with BFA isolated from gAT, scAT, and mAT. (D) CD8+ (top) and CD4+ (bottom) T cells isolated from the mAT. Left: representative plots of CD44 and CD62L expression, Right: representative histograms of CD69 expression on CD44+CD62L− (red) or CD44−CD62L+ (blue) T cells. (E) Numbers of central memory (Tcm: CD44+CD62L+), effector memory (Tem: CD44+CD62L−CD69−) and resident memory (Trm: CD44+CD62L−CD69+) CD8+ and CD4+ T cells per gram of gAT, scAT, and mAT. (F) Conjoined pairs of naïve CD45.1 and CD45.2 congenic C57BL/6 mice were analyzed 6 weeks after parabiosis surgery to quantify the origin of CD8+ (left) and CD4+ (right) memory T cell subsets (described in (E)) in the spleen (Spl), mLN, siLP, and mAT. The percentage of cells originating from host (black bars) or donor (white bars) animals is shown. nd not detected. (G–K) mAT isolated from healthy rhesus macaques was analyzed by flow cytometry. (G) Representative plot (gated on CD3+CD8+ T cells) indicating the gating strategy for naïve (CD95−CD28lo), central memory or stem cell memory (Tcm/scm) (CD95+CD28+), and Tem/rm (CD95+CD28−) T cells. (H) Representative plots showing CD8+ (left) and CD4+ (right) naïve, Tcm/scm, and Tem/rm T cells. (I) Representative histograms of CD69 expression on CD8+ (left) and CD4+ (right) naïve (shaded) and Tem/rm T cells (blue). (J) Frequencies of CD8+ (left) and CD4+ (right) memory T cell subsets from (HI). (K) Representative plots (gated on CD3+ T cells) from concatenation of all samples, showing cytokine production by T cell subsets. Numbers in representative plots indicate mean±SD. In all bar graphs, error bars represent standard deviation. Data are representative of at least 2 experiments with at least 4 mice, 3 pairs of parabiotic animals, or a total of 3 rhesus macaques. See Figure S1.

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: (A–E) ATs from naïve C57BL/6 mice were analyzed by flow cytometry. (A) Representative plots of transcription factor expression or cytokine production by CD8+ or CD4+ T cells from the mAT after PMA/ionomycin restimulation with BFA. (B) Numbers of CD8+ T cells and non-Treg CD4+ T cells from the mAT producing the indicated cytokine. (C) Frequencies of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells after PMA/ionomycin restimulation with BFA isolated from gAT, scAT, and mAT. (D) CD8+ (top) and CD4+ (bottom) T cells isolated from the mAT. Left: representative plots of CD44 and CD62L expression, Right: representative histograms of CD69 expression on CD44+CD62L− (red) or CD44−CD62L+ (blue) T cells. (E) Numbers of central memory (Tcm: CD44+CD62L+), effector memory (Tem: CD44+CD62L−CD69−) and resident memory (Trm: CD44+CD62L−CD69+) CD8+ and CD4+ T cells per gram of gAT, scAT, and mAT. (F) Conjoined pairs of naïve CD45.1 and CD45.2 congenic C57BL/6 mice were analyzed 6 weeks after parabiosis surgery to quantify the origin of CD8+ (left) and CD4+ (right) memory T cell subsets (described in (E)) in the spleen (Spl), mLN, siLP, and mAT. The percentage of cells originating from host (black bars) or donor (white bars) animals is shown. nd not detected. (G–K) mAT isolated from healthy rhesus macaques was analyzed by flow cytometry. (G) Representative plot (gated on CD3+CD8+ T cells) indicating the gating strategy for naïve (CD95−CD28lo), central memory or stem cell memory (Tcm/scm) (CD95+CD28+), and Tem/rm (CD95+CD28−) T cells. (H) Representative plots showing CD8+ (left) and CD4+ (right) naïve, Tcm/scm, and Tem/rm T cells. (I) Representative histograms of CD69 expression on CD8+ (left) and CD4+ (right) naïve (shaded) and Tem/rm T cells (blue). (J) Frequencies of CD8+ (left) and CD4+ (right) memory T cell subsets from (HI). (K) Representative plots (gated on CD3+ T cells) from concatenation of all samples, showing cytokine production by T cell subsets. Numbers in representative plots indicate mean±SD. In all bar graphs, error bars represent standard deviation. Data are representative of at least 2 experiments with at least 4 mice, 3 pairs of parabiotic animals, or a total of 3 rhesus macaques. See Figure S1.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Flow Cytometry, Expressing, Isolation, Standard Deviation

(A–B) Mice were orally infected with Yptb ΔyopM. >4 weeks post-infection, Tem and Trm memory CD8+ T cells were sorted for gene expression analysis by RNA-Seq. (A) Numbers of genes with a ≥2 fold increase (red) or decrease (blue) in expression levels between the indicated populations. (B) Pathway analysis was performed and gene pathways were organized into clusters, represented here as clustergrams showing gene pathways differentially regulated between Trm cells in the siLP and mAT. (C–G) Mice infected with Yptb ΔyopM were analyzed at >4 weeks post-infection. (C) Ki67 expression by Tem and Trm YopE:Kb+CD8+ T cells was determined by flow cytometric analysis. Left hand plots show YopE:Kb+ expression on CD8+ T cells. Center plots show CD69 expression on YopE:Kb+CD8+ cells. Right hand plots show Ki67 expression on Tem and Trm cells. Bar graphs show frequencies of Ki67+YopE:Kb+CD8+ Tem (left) and Trm (right) cells. (D) Representative Vybrant DyeCycle Violet staining of YopE:Kb+CD44+CD8+ T cells from the siLP (blue), spleen (green), and mAT (red). Bar graphs show the percentage of YopE:Kb+CD44+CD8+ Tem and Trm cells in the indicated cell cycle stages in the siLP (left), spleen (center), and mAT (right). (E) >4 weeks post-infection with the Yptb ΔyopM, mice received BrdU for 12 days. The percentage of BrdU+YopE:Kb+CD44+ CD8+ T cells found in the intraepithelial lymphocytes (IEL), lungs, salivary gland (SG), siLP, liver, mAT, and gAT is quantified in the bar graph. (F) >4 weeks post-infection, cells from the siLP, spleen, and mAT were incubated with Bodipy FL C16. Representative histogram indicates Bodipy uptake in YopE:Kb+CD8+ Trm cells (red) or YopE:Kb+CD8+ Tem cells (blue) from the siLP (open) and mAT (shaded). Bar graphs show the quantification of the bodipy MFI. (Student’s t test). (G) >4 weeks post-infection, cells from the siLP, spleen, and mAT were incubated with Mitotracker Deep Red. Representative histograms indicate Mitotracker Deep Red staining in YopE:Kb+CD8+ Trm cells (red) or YopE:Kb+CD8+ Tem cells (blue) from the siLP (open histogram) and mAT (shaded histograms). Bar graphs show the quantification of the Mitotracker Deep Red MFI. Numbers in representative plots indicate mean±SD. Error bars in all graphs represent mean±SD. Data are representative of at least 2 experiments with ≥3 mice per group. One way Anova adjusted for multiple comparisons. **p<0.01, ****p<0.0001 See Figure S3.

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: (A–B) Mice were orally infected with Yptb ΔyopM. >4 weeks post-infection, Tem and Trm memory CD8+ T cells were sorted for gene expression analysis by RNA-Seq. (A) Numbers of genes with a ≥2 fold increase (red) or decrease (blue) in expression levels between the indicated populations. (B) Pathway analysis was performed and gene pathways were organized into clusters, represented here as clustergrams showing gene pathways differentially regulated between Trm cells in the siLP and mAT. (C–G) Mice infected with Yptb ΔyopM were analyzed at >4 weeks post-infection. (C) Ki67 expression by Tem and Trm YopE:Kb+CD8+ T cells was determined by flow cytometric analysis. Left hand plots show YopE:Kb+ expression on CD8+ T cells. Center plots show CD69 expression on YopE:Kb+CD8+ cells. Right hand plots show Ki67 expression on Tem and Trm cells. Bar graphs show frequencies of Ki67+YopE:Kb+CD8+ Tem (left) and Trm (right) cells. (D) Representative Vybrant DyeCycle Violet staining of YopE:Kb+CD44+CD8+ T cells from the siLP (blue), spleen (green), and mAT (red). Bar graphs show the percentage of YopE:Kb+CD44+CD8+ Tem and Trm cells in the indicated cell cycle stages in the siLP (left), spleen (center), and mAT (right). (E) >4 weeks post-infection with the Yptb ΔyopM, mice received BrdU for 12 days. The percentage of BrdU+YopE:Kb+CD44+ CD8+ T cells found in the intraepithelial lymphocytes (IEL), lungs, salivary gland (SG), siLP, liver, mAT, and gAT is quantified in the bar graph. (F) >4 weeks post-infection, cells from the siLP, spleen, and mAT were incubated with Bodipy FL C16. Representative histogram indicates Bodipy uptake in YopE:Kb+CD8+ Trm cells (red) or YopE:Kb+CD8+ Tem cells (blue) from the siLP (open) and mAT (shaded). Bar graphs show the quantification of the bodipy MFI. (Student’s t test). (G) >4 weeks post-infection, cells from the siLP, spleen, and mAT were incubated with Mitotracker Deep Red. Representative histograms indicate Mitotracker Deep Red staining in YopE:Kb+CD8+ Trm cells (red) or YopE:Kb+CD8+ Tem cells (blue) from the siLP (open histogram) and mAT (shaded histograms). Bar graphs show the quantification of the Mitotracker Deep Red MFI. Numbers in representative plots indicate mean±SD. Error bars in all graphs represent mean±SD. Data are representative of at least 2 experiments with ≥3 mice per group. One way Anova adjusted for multiple comparisons. **p<0.01, ****p<0.0001 See Figure S3.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Infection, Expressing, RNA Sequencing Assay, Staining, Incubation

(A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or vehicle control (ctrl.) and evaluated by flow cytometry 1 hour post-injection. (A) Representative histogram shows expression of CD69 on YopE:Kb+CD8+ T cells after vehicle control (dotted line) or peptide (gray shading) injection (left) and MFI of CD69 in the indicated organs after the indicated treatment is quantified (right). (B) Representative plots show IFN-γ and TNF-α production by YopE:Kb+CD8+ T cells 1 hour after vehicle control (left) or peptide (right) injection in the indicated organs. Frequencies of IFN-γ+ TNF-α+ YopE:Kb+ CD8+ T cells in the indicated organs are shown in the bar graph. (C) Mice were infected orally with a fluorescent reporter strain of T. gondii. 6 weeks post-infection, mice were injected i.v. with MHC-I and MHC-II peptides or vehicle control and analyzed by flow cytometry 1 hour post injection. Representative plots show IFN-γ and TNF-α in CD44+CD8+ T cells in the indicated organ. Bar graphs show the frequencies of IFN-γ+TNF-α+ cells within the CD44+ CD8+ T cells. (D–E) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69-77 peptide or an equal volume of vehicle control and evaluated by flow cytometry at 1 and 4 hours post-injection. (D) Representative plots show neutrophils in the indicated organs (gated on live CD45+TCRβ−Siglec F−NK1.1−B220− cells) 4 hours after injection. Bar graph shows frequency of neutrophils at 1 and 4 hours post-injection in the siLP and mAT. (E) Representative plots show monocytes (gated on live CD45+TCR-β−Siglec F−NK1.1−B220−CD11b+Ly-6G−CD64+CCR2+ cells) 4 hours after injection in the indicated organs. Bar graph shows the frequency of monocytes at 1 and 4 hours post-injection in the siLP and mAT. Numbers in representative plots indicate mean±SD. Error bars in all bar graphs represent standard deviation and statistics are calculated using one way Anova adjusted for multiple comparisons. Data are representative of at least 2 experiments with 2–6 mice per group. ns not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S5.

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: (A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or vehicle control (ctrl.) and evaluated by flow cytometry 1 hour post-injection. (A) Representative histogram shows expression of CD69 on YopE:Kb+CD8+ T cells after vehicle control (dotted line) or peptide (gray shading) injection (left) and MFI of CD69 in the indicated organs after the indicated treatment is quantified (right). (B) Representative plots show IFN-γ and TNF-α production by YopE:Kb+CD8+ T cells 1 hour after vehicle control (left) or peptide (right) injection in the indicated organs. Frequencies of IFN-γ+ TNF-α+ YopE:Kb+ CD8+ T cells in the indicated organs are shown in the bar graph. (C) Mice were infected orally with a fluorescent reporter strain of T. gondii. 6 weeks post-infection, mice were injected i.v. with MHC-I and MHC-II peptides or vehicle control and analyzed by flow cytometry 1 hour post injection. Representative plots show IFN-γ and TNF-α in CD44+CD8+ T cells in the indicated organ. Bar graphs show the frequencies of IFN-γ+TNF-α+ cells within the CD44+ CD8+ T cells. (D–E) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69-77 peptide or an equal volume of vehicle control and evaluated by flow cytometry at 1 and 4 hours post-injection. (D) Representative plots show neutrophils in the indicated organs (gated on live CD45+TCRβ−Siglec F−NK1.1−B220− cells) 4 hours after injection. Bar graph shows frequency of neutrophils at 1 and 4 hours post-injection in the siLP and mAT. (E) Representative plots show monocytes (gated on live CD45+TCR-β−Siglec F−NK1.1−B220−CD11b+Ly-6G−CD64+CCR2+ cells) 4 hours after injection in the indicated organs. Bar graph shows the frequency of monocytes at 1 and 4 hours post-injection in the siLP and mAT. Numbers in representative plots indicate mean±SD. Error bars in all bar graphs represent standard deviation and statistics are calculated using one way Anova adjusted for multiple comparisons. Data are representative of at least 2 experiments with 2–6 mice per group. ns not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S5.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Infection, Injection, Flow Cytometry, Expressing, Standard Deviation

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

(A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or vehicle control (ctrl.) and evaluated by flow cytometry 1 hour post-injection. (A) Representative histogram shows expression of CD69 on YopE:Kb+CD8+ T cells after vehicle control (dotted line) or peptide (gray shading) injection (left) and MFI of CD69 in the indicated organs after the indicated treatment is quantified (right). (B) Representative plots show IFN-γ and TNF-α production by YopE:Kb+CD8+ T cells 1 hour after vehicle control (left) or peptide (right) injection in the indicated organs. Frequencies of IFN-γ+ TNF-α+ YopE:Kb+ CD8+ T cells in the indicated organs are shown in the bar graph. (C) Mice were infected orally with a fluorescent reporter strain of T. gondii. 6 weeks post-infection, mice were injected i.v. with MHC-I and MHC-II peptides or vehicle control and analyzed by flow cytometry 1 hour post injection. Representative plots show IFN-γ and TNF-α in CD44+CD8+ T cells in the indicated organ. Bar graphs show the frequencies of IFN-γ+TNF-α+ cells within the CD44+ CD8+ T cells. (D–E) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69-77 peptide or an equal volume of vehicle control and evaluated by flow cytometry at 1 and 4 hours post-injection. (D) Representative plots show neutrophils in the indicated organs (gated on live CD45+TCRβ−Siglec F−NK1.1−B220− cells) 4 hours after injection. Bar graph shows frequency of neutrophils at 1 and 4 hours post-injection in the siLP and mAT. (E) Representative plots show monocytes (gated on live CD45+TCR-β−Siglec F−NK1.1−B220−CD11b+Ly-6G−CD64+CCR2+ cells) 4 hours after injection in the indicated organs. Bar graph shows the frequency of monocytes at 1 and 4 hours post-injection in the siLP and mAT. Numbers in representative plots indicate mean±SD. Error bars in all bar graphs represent standard deviation and statistics are calculated using one way Anova adjusted for multiple comparisons. Data are representative of at least 2 experiments with 2–6 mice per group. ns not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S5.

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: (A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or vehicle control (ctrl.) and evaluated by flow cytometry 1 hour post-injection. (A) Representative histogram shows expression of CD69 on YopE:Kb+CD8+ T cells after vehicle control (dotted line) or peptide (gray shading) injection (left) and MFI of CD69 in the indicated organs after the indicated treatment is quantified (right). (B) Representative plots show IFN-γ and TNF-α production by YopE:Kb+CD8+ T cells 1 hour after vehicle control (left) or peptide (right) injection in the indicated organs. Frequencies of IFN-γ+ TNF-α+ YopE:Kb+ CD8+ T cells in the indicated organs are shown in the bar graph. (C) Mice were infected orally with a fluorescent reporter strain of T. gondii. 6 weeks post-infection, mice were injected i.v. with MHC-I and MHC-II peptides or vehicle control and analyzed by flow cytometry 1 hour post injection. Representative plots show IFN-γ and TNF-α in CD44+CD8+ T cells in the indicated organ. Bar graphs show the frequencies of IFN-γ+TNF-α+ cells within the CD44+ CD8+ T cells. (D–E) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69-77 peptide or an equal volume of vehicle control and evaluated by flow cytometry at 1 and 4 hours post-injection. (D) Representative plots show neutrophils in the indicated organs (gated on live CD45+TCRβ−Siglec F−NK1.1−B220− cells) 4 hours after injection. Bar graph shows frequency of neutrophils at 1 and 4 hours post-injection in the siLP and mAT. (E) Representative plots show monocytes (gated on live CD45+TCR-β−Siglec F−NK1.1−B220−CD11b+Ly-6G−CD64+CCR2+ cells) 4 hours after injection in the indicated organs. Bar graph shows the frequency of monocytes at 1 and 4 hours post-injection in the siLP and mAT. Numbers in representative plots indicate mean±SD. Error bars in all bar graphs represent standard deviation and statistics are calculated using one way Anova adjusted for multiple comparisons. Data are representative of at least 2 experiments with 2–6 mice per group. ns not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S5.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Infection, Injection, Flow Cytometry, Expressing, Standard Deviation

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

DATA AND SOFTWARE AVAILABILITY

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse α4β7 (DATK32) eBioscience Cat# 12-5887-82 Anti-mouse B220 (RA3-6B2) BD Biosciences Cat# 562290 Anti-mouse CCR2(FAB5538P) R&D Systems Cat# FAB5538P Anti-mouse CCR7 (4B12) Biolegend Cat# 120107 Anti-mouse CCR9 (CW1.2) eBioscience Cat# 12-1991-82 Anti-mouse CD3 (17A2) eBioscience Cat# 47-0032-82 Anti-mouse CD4 (RM4-5) Biolegend Cat# 100548 Anti-mouse CD8β (H35–17.2) eBioscience Cat# 25-0083-82 Anti-mouse CD11a (M17/4) eBioscience Cat# 12-0111-82 Anti-mouse CD11b (M1/70) BD Biosciences Cat# 563015 Anti-mouse CD11c (N418) Biolegend Cat# 117336 Anti-mouse CD44 (IM7) eBioscience Cat# 25-0441-82 Anti-mouse CD45 (30-F11) eBioscience Cat# 47-0451-82 Anti-mouse CD45.1 (A20) Biolegend Cat# 110737 Anti-mouse CD45.2 (104) Biolegend Cat# 109837 Anti-mouse CD62L (MEL-14) eBioscience Cat# 11-0621-85 Anti-mouse CD69 (H1.2F3) eBioscience Cat# 12-0691-83 Anti-mouse CD90.2 (30-H12) Biolegend Cat# 105331 Anti-mouse CD103 (2E7) eBioscience Cat# 46-1031-82 Anti-mouse CD122 (TMb1) eBioscience Cat# 46-1222-82 Anti-mouse CD127 (A7R34) Biolegend Cat# 135025 Anti-mouse Foxp3 (FJK-16S) eBioscience Cat# 17-5773-82 Anti-mouse γδTCR (GL3) BD Biosciences Cat# 563532 Anti-mouse GATA-3 (L50-823) BD Biosciences Cat# 563349 Anti-mouse IFN-γ (XMG1.2) eBioscience Cat# 48-7311-82 Anti-mouse IL-5 (TRFK5) Fisher Scientific Cat# 554395 Anti-mouse IL-13 (13A) eBioscience Cat# 53-7133-82 Anti-mouse IL-17A (17B7) Biolegend Cat# 506910 Anti-mouse Ki67 (SolA15) eBioscience Cat# 48-5698-82 Anti-mouse KLRG1 (2F1) eBioscience Cat# 12-5893-82 Anti-mouse Ly-6C (HK1.5) Biolegend Cat# 128035 Anti-mouse Ly-6G (IA8) BD Biosciences Cat# 562700 Anti-mouse MHC-II (M5/114.15.2) eBioscience Cat# 56-5321-82 Anti-mouse NK1.1 (PK136) BD Biosciences Cat# 562864 Anti-mouse Siglec F (E50-2440) BD Biosciences Cat# 562757 Anti-mouse T-bet (4B10) Biolegend Cat# 644816 Anti-mouse TCRβ (H57–597) eBioscience Cat# 47-5961-82 Anti-mouse TNF-α (MP6-XT22) BD Biosciences Cat# 558000 Anti-human CD3 (SP34-2) BD Biosciences Cat# 558124 Anti-human CD4 (OKT4) eBioscience Cat# 93-0048-42 Anti-human CD8 (SK1) eBioscience Cat# 46-0087-42 Anti-human CD28 (CD28.2) Beckman Coulter Cat# 6607111 Anti-human CD69 (FN50) BD Biosciences Cat# 557745 Anti-human CD95 (DX2) Biolegend Cat# 305610 Anti-human CD103 (HML-1) Beckman Coulter Cat# IM 1856 Anti-human IFN-γ (B27) BD Biosciences Cat# 561024 Anti-human IL-13 (JES10-5A2) BD Biosciences Cat# 554571 Anti-human TNF-α (MAb11) eBiosciences Cat# 25-7349-82 Anti-mouse CD4 (GK1.1) (depletion) BioXcell Cat# BE0003-1 Anti-mouse CD8α (2.43) (depletion) BioXcell Cat# BE0061 Bacterial and Virus Strains Yersinia pseudotuberculosis (32777) Laboratory of I.E.B.

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray